Journal: NPJ Breast Cancer

Article Title: NDRG4 promoter hypermethylation is a mechanistic biomarker associated with metastatic progression in breast cancer patients

doi: 10.1038/s41523-019-0106-x

Figure Lengend Snippet: NDRG4 depletion in breast tumor cells increases lymph node adhesion and cell migration toward VN. a NDRG4 expression meta-analysis in human breast cancer cell lines using GOBO application . Breast cancer subtypes can be identified by different colors: red = Basal A, gray = Basal B and blue = Luminal cells. b Analysis of NDRG4 mRNA expression levels in MCF-7 cells transfected with NDRG4-shRNAs or scramble control (shSCR). Expression levels are relative to wild type cells and normalized to hydroxymethylbilane synthase (HMBS) gene. Error bars represent SEM of biological replicates ( n = 5). *** p < 0.001, ns = not significant, by one-way ANOVA. c Western blot analysis of NDRG4 expression in cytoplasmic extracts of MCF-7 shNDRG4s or shSCRs cells, using antibodies against NDRG4 and Actin. MCF-7 cells express all the three isoforms of NDRG4: 37 kDa (NDRG4-B), 39 kDa (NDRG4-B var ) and 41 kDa isoform (NDRG4-H). For simplicity, results obtained for two independent shNDRG4 clones were grouped and presented as the shNDRG4 group. ( d , left) Representative images of adherent red fluorescent MCF-7 cells on frozen rat lymph node sections. d , e Quantification of adherent MCF7 ( d ) and T47D ( e ) breast tumor cells in lymph nodes sections (right, n = 4 independent experiments for MCF-7 and 3 for T47D, ** p < 0.01 by two-tailed t test). f Stationary adhesion assays showed that NDRG4 knockdown promotes ‘adhesive switch’ between FN and VN. Error bars represent SEM of biological replicates ( n = 4). Cells do not adhere to albumin control (BSA, data not shown). * p < 0.01, ** p < 0.01, ns = not significant by two-tailed t tests. g , h Haptotactic cell migration toward VN was analyzed by transwell migration assay in MCF-7 ( g ) and T47D ( h ) cell lines. Error bars represent SEM of biological replicates ( n = 4). *** p < 0.001 by two-tailed t tests

Article Snippet: For stable transfection of NDRG4 shRNA into MCF-7 and T47D breast tumor cells, the pGFP-V-RS vector containing shRNA inserts which targeted the 3′-unttranslated region of NDRG4 (TRCN0000134583 and TRCN0000137216) was purchased (Origene).

Techniques: Migration, Expressing, Transfection, Western Blot, Clone Assay, Two Tailed Test, Transwell Migration Assay

Journal: NPJ Breast Cancer

Article Title: NDRG4 promoter hypermethylation is a mechanistic biomarker associated with metastatic progression in breast cancer patients

doi: 10.1038/s41523-019-0106-x

Figure Lengend Snippet: NDRG4 knockdown promotes clustering of β1-integrin at the leading edge of T47D cells. Representative confocal images of β1 integrin subunit (MAB1965, green) at the ventral cell surface of VN-adherent T47D shNDRG4 or shSCR cells

Article Snippet: For stable transfection of NDRG4 shRNA into MCF-7 and T47D breast tumor cells, the pGFP-V-RS vector containing shRNA inserts which targeted the 3′-unttranslated region of NDRG4 (TRCN0000134583 and TRCN0000137216) was purchased (Origene).

Techniques:

Journal: Asian Pacific Journal of Cancer Prevention : APJCP

Article Title: Unexpected Lower Expression of Oncoprotein Gankyrin in Drug Resistant ABCG2 Overexpressing Breast Cancer Cell Lines

doi: 10.22034/APJCP.2017.18.12.3413

Figure Lengend Snippet: The Expression of ABCG2 at mRNA Level in MCF-7 Derived Breast Cancer Cell Lines. ABCG2 mRNA level is compared between drug resistance cell line MCF-7/MX and its parental non-resistance MCF-7 cells. Real-time RT-PCR analysis was performed on total RNA extracted from cells. Values were normalized to the β-actin content of samples and expressed as mean ±SEM (n = 3). Statistical significance was calculated by one-way Analysis of variance (ANOVA) and Tukey’s post-hoc; ***p < 0.001 compared with the non-resistance parental cell line.

Article Snippet: ABCG2 overexpressing cell line MCF-7/MX and MDR1 overexpressing cell line MCF-7/ADR were kindly provided by Professor Herman Lage (Molecular pathology department, Charite Campus Mitte, Berlin, Germany).

Techniques: Expressing, Derivative Assay, Quantitative RT-PCR

Journal: ACS Omega

Article Title: Nanohydroxyapatite-, Gelatin-, and Acrylic Acid-Based Novel Dental Restorative Material

doi: 10.1021/acsomega.0c03125

Figure Lengend Snippet: Effect of NDRM on the colony formation against normal breast epithelial cells (fR2): (a) control, (b) camptothecin, (c) hydroxyapatite nanopowder, and (d) NDRM.

Article Snippet: The normal human breast epithelial cell line (fR2) was obtained from the European Collection of Authenticated Cell Cultures (ECACC), U.K.

Techniques: Control

Journal: ACS Omega

Article Title: Nanohydroxyapatite-, Gelatin-, and Acrylic Acid-Based Novel Dental Restorative Material

doi: 10.1021/acsomega.0c03125

Figure Lengend Snippet: Effect of NDRM on the colony formation against normal breast epithelial cells (fR2).

Article Snippet: The normal human breast epithelial cell line (fR2) was obtained from the European Collection of Authenticated Cell Cultures (ECACC), U.K.

Techniques: